Measurement

Part:BBa_K602013

Designed by: Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh   Group: iGEM11_Osaka   (2011-10-05)


E. coli RecA promoter with CrtEBI reporter

Lycopene biosynthesis genes CrtEBI (with RBS) under control of the RecA promoter. The E. coli RecA promoter is triggered by DNA damage, and should result in the production of lycopene turning the cells orange-red.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1710
    Illegal NgoMIV site found at 1840
    Illegal AgeI site found at 925
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None